QuTEM AAV cryo

Adeno-associated viral vectors

Adeno-associated virus (AAV)-based gene therapies have shown great promise in clinical trials, offering hope for treating a variety of genetic disorders. The efficacy of these therapies depends, among many factors, on the ratio of full to empty capsids, a critical quality attribute which can be clearly evaluated using cryogenic Transmission Electron Microscopy (cryoTEM). 

By visualizing each AAV particle, TEM can differentiate between fully filled, partially filled, and empty particles, which is crucial information for understanding treatment efficacy and immunogenicity. Compared to orthogonal methods, TEM analysis is relatively unaffected by the presence of proteins, debris, and aggregation, allowing for accurate particle classification and ratio calculations.  

Analyses

At QuTEM, we generally offer the following analyses for AAV particles.

Example reports

Download an example report that includes:

  • Analysis of an AAV sample by cryoTEM, including genome length determination
  • Analysis of an AAV sample by nsTEM
QuTEM AAV ns

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