Modified Vaccinia Ankara Virus

Modified Vaccinia Ankara (MVA) virus is a commonly used viral vector in vaccine development. Its large genome capacity makes it highly valuable in this field. The bioactivity of MVA-based products relies heavily on the viral titer of mature rectangular particles, which possess complete capsid structures and a double lipid membrane. These intricate features of MVAs can be meticulously examined with Transmission Electron Microscopy (TEM). TEM allows for detailed characterization of vector morphology, offering valuable insights into the maturation state of MVA particles.  

The vectors can be classified based on their morphology and can be therefore calculated into subpopulation ratios based on maturation state. This enables a more specific comparison between different batches or lots, offering valuable insights into their composition and efficacy in vaccine development. Moreover, TEM serves as a reliable tool for monitoring the purity and integrity of the product throughout purification and manufacturing stages.